Protein C 标记 (NULL) 抗体

ABIN356540 产品详细信息, 供应商: Log in to see
抗原
适用
标记
4
宿主

3
1
克隆类型
多克隆
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
4
2
1
1
1
1
选项
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免疫原 KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen.
亚型 Ig
特异性 This antibody is specific to Protein-C Tag.
纯化方法 Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
物质类 Tag
背景 Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. This polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.Synonyms: C tag, C-Tag, Protein C Epitope tag
基因ID 5624, 5874
UniProt P04070
应用备注 ELISA: 1/5,000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS containing 0.09 % (W/V) Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
厂商提供的图像
 image for anti-Protein C Tag (NULL) antibody (ABIN356540) anti-Protein C Tag (NULL) antibody
背景 Lichty, Malecki, Agnew, Michelson-Horowitz, Tan: "Comparison of affinity tags for protein purification." in: Protein expression and purification, Vol. 41, Issue 1, pp. 98-105, 2005 (PubMed).

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