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Protein C Tag 抗体

WB, ELISA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1573910
发货至: 中国
  • 抗原 See all Protein C Tag products
    Protein C Tag
    宿主
    • 2
    • 1
    克隆类型
    • 2
    • 1
    多克隆
    标记
    • 3
    This Protein C Tag antibody is un-conjugated
    应用范围
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    特异性
    This Antibody recognizes C-terminal, N-terminal, and internal Protein C tagged fusion proteins.
    纯化方法
    Immunoaffinity chromatography
    免疫原
    Protein C epitope tag peptide CEDQVDPRLIDGK conjugated - KLH
    亚型
    IgG
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4, containing 0.02 %Sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wong, Bienzle, Hayes, Taylor, Wood: "Purification of protein C from canine plasma." in: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).

    Wolf, Reimer, Schuck, Rüder, Gerlach, Müller, Otto, Dörken, Rehm: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).

  • 抗原
    Protein C Tag
    Abstract
    Protein C Tag 产品
    物质类
    Tag
    背景
    Rabbit Anti-Protein C (HPC4)-tag is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% Sodium azide.
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