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CD14 抗体 (AA 9-13, AA 39-44)

Name: CD14 抗体 (AA 9-13, AA 39-44)
Brand: antibodies-online
SKU: ABIN343168
Availability: OutOfStock

CD14 适用: 人 FACS, WB, ELISA, Agon 宿主: 小鼠 Monoclonal biG13 unconjugated

Western Blotting (WB) image for anti-CD14 (CD14) (AA 39-44), (AA 9-13) antibody (ABIN343168)

1 image

(5 references)

产品编号 ABIN343168

发货至: 中国

Datasheet as PDF

抗原 See all CD14 抗体

CD14
抗原表位
16

9

7

6

5

4

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 9-13, AA 39-44
适用
246

47

30

29

28

28

25

24

22

18

15

14

10

9

8

8

8

5

2

2

2

1

1

1
人
宿主
203

64

16

3

1
小鼠
Compatible Secondaries
克隆类型
223

64
单克隆
标记
120

29

22

17

13

6

5

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
应用范围
201

98

60

52

47

45

22

21

20

14

13

13

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Agonist (Agon)

特异性

Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14

交叉反应

Corynebacterium diphtheriae, 牛, 犬, 马, 猪

交叉反应（详细）

CD14 of cattle++, swine ++, dog+ horse+

纯化方法

Protein G affinity column

免疫原

Monocytes of different species and immunoaffinity purified soluble human CD14

克隆位点

BiG13

亚型

IgG1

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CD14 适用: 人, 小鼠, 大鼠, 犬, 绵羊, 兔 WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

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应用备注

20 μg/mL inhibits binding of 0.5 μg/ ml LPS to CD14.Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute with bidistilled water

浓度

1 mg/mL

缓冲液

PBS, pH 7.5

储存液

Without preservative

储存条件

-20 °C,-80 °C
Moyo, Marchi, Steinbach: "Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression." in: Immunology, Vol. 139, Issue 4, pp. 472-83, (2013) (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, (2012) (PubMed).

Kato, Ogasawara, Homma, Saito, Matsumoto: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, (2004) (PubMed).

Berner, Fürll, Stelter, Dröse, Müller, Schütt: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, (2002) (PubMed).

Vidal, Labéta, Schiffrin, Donnet-Hughes: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, (2001) (PubMed).
抗原

CD14

别名

CD14 (CD14 产品)

别名

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

背景

Background: biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

分子量

~50kDa

基因ID

929

NCBI登录号

NP_000582

UniProt

P08571

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades