SMAD5 抗体 (Middle Region)
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- 抗原 See all SMAD5 抗体
- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
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抗原表位
- AA 248-267, Middle Region
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SMAD5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 5(SMAD5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- IPQIMPSISS RDVQPVAYEE
- 交叉反应 （详细）
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 5(SMAD5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: SMAD family member 5
Protein Name: Mothers against decapentaplegic homolog 5 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human SMAD5(248-267aa IPQIMPSISSRDVQPVAYEE), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product SMAD5 Primary Antibody
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anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 210-464) antibody
SMAD5 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 140-240) antibodySMAD5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibodySMAD5 适用: 人, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3H9 unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 1-209) antibodySMAD5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 105-182) antibodySMAD5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 186-465) antibodySMAD5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (pSer463), (pSer465) antibodySMAD5 适用: 人, 小鼠, 大鼠 WB, IF (cc), IHC (p) 宿主: 兔 Monoclonal 1B8 unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibodySMAD5 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 105-182) antibodySMAD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5E12 unconjugated
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibodySMAD5 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SMAD5 is approximately 1.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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- 抗原
- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
- 别名
- SMAD5 (SMAD5 产品)
- 别名
- DWFC antibody, JV5-1 antibody, MADH5 antibody, Madh5 antibody, SBN antibody, fb67b04 antibody, mad antibody, madh5 antibody, sb:cb400 antibody, smd5 antibody, wu:fb67b04 antibody, SMAD5 antibody, DKFZp468M0619 antibody, 1110051M15Rik antibody, AI451355 antibody, Dwf-C antibody, MusMLP antibody, SMAD family member 5 antibody, SMAD5 antibody, Smad5 antibody, smad5 antibody
- 背景
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Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins(BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.
Synonyms: DKFZp781C1895 antibody|DKFZp781O1323 antibody|Dwfc antibody|hSmad 5 antibody|hSmad5 antibody|JV5 1 antibody|JV5-1 antibody|MAD homolog 5 antibody|MAD mothers against decapentaplegic homolog 5 antibody|MAD, mothers against decapentaplegic homolog 5 antibody|MADH 5 antibody|MADH5 antibody|Mothers against decapentaplegic homolog 5 antibody|Mothers against DPP homolog 5 antibody|MusMLP antibody|SMA and MAD related protein 5 antibody|SMAD 5 antibody|SMAD family member 5 antibody|SMAD mothers against DPP homolog 5 antibody|Smad5 antibody|SMAD5_HUMAN antibody - UniProt
- Q99717
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