MAPK11 抗体 (Mitogen-Activated Protein Kinase 11) (N-Term)

Details for Product anti-MAPK11 Antibody No. ABIN2798348, 供应商: Log in to see
抗原
  • ATMPK11
  • F22L4.10
  • F22L4_10
  • MAP kinase 11
  • zgc:86905
  • P38B
  • P38BETA2
  • PRKM11
  • SAPK2
  • SAPK2B
  • p38-2
  • p38Beta
  • P38b
  • Prkm11
  • Sapk2
  • Sapk2b
  • p38beta
  • p38beta2
  • p38b
  • sapk2
  • prkm11
  • sapk2b
  • mitogen-activated protein kinase 11
  • mapk11
  • ARALYDRAFT_887292
  • MPK11
  • MAPK11
  • Mapk11
  • PTRG_02565
抗原表位
N-Term
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适用
小鼠
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83
48
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1
宿主

119
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克隆类型
多克隆
标记
This MAPK11 antibody is un-conjugated
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应用范围
Immunohistochemistry (IHC), Western Blotting (WB)
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1
选项
Supplier
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Supplier Product No.
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'独立验证'标志
抗原 MAPK11
Lot Number RAY8110713
Method validated Western Blotting
Positive Control primary mouse microglia
Negative Control siRNA knock-down of MAPK11 in primary mouse microglia
Notes Passed. ABIN2798348 specifically recognizes Mapk11 in primary mouse microglia lysates.
Primary Antibody ABIN2798348
Secondary Antibody anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
实验流程
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse MAPK11 Silencer Select siRNA (ThermoFisher Scientific, Sample ID ASO10943, ASO1098J and ASO109CX, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer´s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary rabbit anti-MAPK11 antibody (antibodies-online, ABIN2798348, lot RAY8110713) diluted 1:500 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Experimental Notes The MAPK11 antibody ABIN2798348 reveals a protein of the expected molecular weight of murine Mapk11 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validation Images
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (N-Term) antibody (ABIN2798348) Primary mouse microglia were transfected with Mapk11 siRNA and Mapk11 was revealed us...
亚型 Ig Fraction
别名 Mapk11 (MAPK11 Antibody 摘要)
分子量 41397 Da
途径 MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Response to Water Deprivation, Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Hepatitis C, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
应用备注 Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
说明

Rabbit Anti-Mouse Mouse Mapk11 (N-term) Antibody, 400 μl

限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
储存条件 4 °C,-20 °C
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