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HYAL1 抗体 (Hyaluronidase-1) (N-Term)

Details for Product anti-HYAL1 Antibody No. ABIN2784343, 供应商: Log in to see
抗原
  • HYAL-1
  • LUCA1
  • NAT6
  • Hya1
  • Hyal-1
  • hyaluronoglucosaminidase 1
  • HYAL1
  • Hyal1
Alternatives
anti-人 HYAL1 抗体 for Immunofluorescence (fixed cells)
抗原表位
N-Term
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15
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10
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2
1
1
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适用
牛, 犬, 豚鼠, 马, 人, 小鼠, 兔, 大鼠
76
39
27
6
4
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2
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宿主

82
23
8
克隆类型
多克隆
标记
This HYAL1 antibody is un-conjugated
8
8
5
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4
1
1
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应用范围
Immunohistochemistry (IHC), Western Blotting (WB)
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53
37
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7
6
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3
1
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1
Supplier
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Supplier Product No.
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Available images

'独立验证'标志
抗原 HYAL1 antibody - N-terminal region
Lot Number QC23783
Method validated Immunohistochemistry
Positive Control Testes of Hyal1 +/+ mouse
Negative Control Testes of Hyal1 -/- mouse
Notes ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.
Primary Antibody ABIN2784343
Secondary Antibody Goat-anti-rabbit AF568
实验流程
  • Deparafinize and rehydrate:
    • xylene (I & II) – 2x 5min
    • 100% Ethanol (I & II) – 2x 5min
    • 95% Ethanol (I & II) – 2x 3min
    • 70% Ethanol (I) – 3min
    • 50% Ethanol (I) – 3min
    • ddH2O – 3min
  • Antigen retrieval:
    • Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
    • Incubate at 95°C for 20min.
    • Cool down at RT for 20min.
    • Wash twice with PBS for 5min.
  • Immunostaining:
    • Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
    • Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
    • Incubate at 4°C overnight.
    • Wash slides three times with TBST 5min.
    • Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
    • Incubate at RT for 1h.
    • Wash slides in TBST - 1x 5min at RT.
    • Wash three times with H2O for 5min.
    • Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
    • Wash twice with H2O for 5min.
    • Mounted with ProLong Gold (ThermoFisher, P36931) solution.
Validation Images
Immunohistochemistry validation image for anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody (ABIN2784343) Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as desc...
免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1
Blocking Peptide Blocking peptide for this product available: ABIN978363
序列 TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD
预测反应 Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%
产品特性 This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Affinity Purified
别名 HYAL1 (HYAL1 Antibody 摘要)
背景 HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
Protein Interaction Partner: UBC, COL2A1,
Protein Size: 435
分子量 45 kDa
基因ID 3373
NCBI登录号 NM_153282, NP_695014
UniProt Q12794
研究领域 Cancer, Extracellular Matrix
途径 Glycosaminoglycan Metabolic Process
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 435 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-HYAL1 抗体 (Hyaluronidase-1) (N-Term) (ABIN2784343) WB Suggested Anti-HYAL1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
背景 Poola, Abraham, Marshalleck, Yue, Lokeshwar, Bonney, Dewitty: "Molecular risk assessment for breast cancer development in patients with ductal hyperplasias." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 4, pp. 1274-80, 2008 (PubMed).