Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
This antibody reacts to MTA2 / PID. No cross response to MTA1.
纯化方法
Purified
免疫原
Rabbit polyclonal PID antibody was raised against a synthetic peptide corresponding to amino acids 652 to 668 of human PID/MTA2 (6), which differ from the mouse sequence by one amino acid (7).
Western Blot: 0.5 to 1 μg/mL, a 75 kDa band can be detected. Tissue Expression: Widely expressed, located in the nucleus. Positive Control: HeLa cell lysate. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
Phosphate buffered saline with 0.02 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
抗原
MTA2
(Metastasis Associated 1 Family, Member 2 (MTA2))
MTA1L1 antibody, PID antibody, fd61b05 antibody, wu:fd61b05 antibody, wu:fi29g02 antibody, zgc:63487 antibody, pid antibody, mta1l1 antibody, MTA2 antibody, DKFZp470E0911 antibody, AW550797 antibody, Mata1l1 antibody, Mta1l1 antibody, mmta2 antibody, metastasis associated 1 family member 2 antibody, metastasis associated 1 family, member 2 antibody, metastasis-associated gene family, member 2 antibody, metastasis associated 1 family member 2 L homeolog antibody, MTA2 antibody, mta2 antibody, Mta2 antibody, mta2.L antibody
背景
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex (6 - 8). PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis (6). PID/MTA2 is ubiquitously expressed in human tissues (8).Synonyms: MTA1-L1, MTA1L1, Metastasis-associated 1-like 1, Metastasis-associated protein MTA2, PID, p53 target protein in deacetylase complex