Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
特异性
This JARID2 antibody is useful for Immunocytochemistry/Immunofluorescence (staining is nuclear) and Western blot. By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. This antibody has also been reported to work in Chromatin Immunoprecipitation (ChIP), see PMID: 22396653.
纯化方法
Aff - Purified
免疫原
A synthetic peptide made to an N-terminal portion of the human JARID2 or jumonji protein sequence (AA 1-100).
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200 , Western Blot 2µg/mL, Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
限制
仅限研究用
状态
Liquid
浓度
1.83 mg/mL
缓冲液
Tris-glycine 150mM NaCl with 0.05% Sodium Azide as a preservative.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Repeated freeze/thaw cycles
储存条件
-20 °C
储存方法
Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C. Compromise the integrity of the antiserum.
抗原
JARID2
(Jumonji, AT Rich Interactive Domain 2 (JARID2))