His 标记 抗体 (Biotin)

ABIN1842272 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
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宿主

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克隆类型
多克隆
标记
Biotin
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应用范围
Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 His epitope tag peptide HHHHHH conjugated to KLH.
序列 HHHHHH
亚型 IgG
特异性 His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.
产品特性 His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. It is suitable for detecting the expression level of His tagged proteins. His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. The antibody recognizes fusion protein with His tag which is located at either C-terminal, or internal regions of the protein. It is suitable for detecting the expression level of His tagged proteins.
纯化方法 Immunoaffinity chromatography
物质类 Tag
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Western blot: 0. 2-1 µg/mL
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
浓度 0.5 mg/mL
缓冲液 lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
有引用在: Dong, Blissard: "Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes." in: Journal of virology, Vol. 86, Issue 18, pp. 9617-28, 2012 (PubMed).

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