Keratin Basic 抗体
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- 抗原 See all Keratin Basic 抗体
- Keratin Basic
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适用
- 人, 大鼠, Cow, Amphibian
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody represents an excellent marker for distinguishing carcinomas from non-epithelial tumors. Polypeptides Reacting: Mr 52 500-Mr 68 000 keratins (type II keratins K1-K8, formerly also designated Cytokeratins 1-8) of Human epithelial cells. Tumors Specifically Detected: All epithelium-derived neoplasms. Tested Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, Bovine BMGE+H, BMGE-H, MDBK.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Bovine, Rat, Amphibia.
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Cytoskeletal proteins from cultured Human MCF-7 cells
- 克隆位点
- Ks pan 1-8
- 亚型
- IgG2a
- Top Product
- Discover our top product Primary Antibody
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anti-Keratin Basic antibody
适用: 人, 大鼠 WB, IF, FACS, IHC (p), ELISA 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal SPM591 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, Non-Human Primate WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal AE3 unconjugated
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
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- 应用备注
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Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and cytological material: 1: 10, 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in 1 mL distilled water
- 缓冲液
- Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- 抗原
- Keratin Basic
- Abstract
- Keratin Basic 产品
- 背景
- Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
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