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Recombinant Keratin Basic 抗体

适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
产品编号 ABIN4949251
发货至: 中国
  • 抗原 See all Keratin Basic 抗体
    Keratin Basic
    抗体类型
    Recombinant Antibody
    适用
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    宿主
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    克隆类型
    • 22
    单克隆
    标记
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    非结合性
    应用范围
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    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein A affinity chromatography
    免疫原
    Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
    克隆位点
    KRTH-1576R
    亚型
    IgG kappa
    Top Product
    Discover our top product Primary Antibody
  • 应用备注
    Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Keratin Basic
    别名
    Basic Cytokeratin (Keratin Basic 产品)
    背景
    This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40  kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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