小鼠 anti-人 IgE Antibody
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- 抗原 See all IgE products
- IgE
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
- 纯化方法
- Precipitation methods and ion exchange chromatography
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Purified human IgE
- 克隆位点
- BE5
- 亚型
- IgG1
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Mouse anti-Human IgE antibody (FITC)
FACS Monoclonal BE5 IgG1 FITC
Mouse anti-Human IgE antibody (PE)FACS Monoclonal BE5 IgG1 PE
Mouse anti-Human IgE (Fc Region) antibodyELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
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- 应用备注
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ELISA. Flow Cytometry: 5 - 10 μg/mL.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 4-8 °C for up to one month or at -28 °C for longer.
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- 抗原
- IgE
- Abstract
- IgE 产品
- 背景
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.Synonyms: Immunoglobulin E human
- 分子量
- 180 kDa
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