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- 抗原 See all RAD52 抗体
- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
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抗原表位
- AA 360-375
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAD52 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 序列
- Human RAD52, 419 aa, predicted MW 46.3 kDa: 1 msgteeailg grdshpaagg gsvlcfgqcq ytaeeyqaiq kalrqrlgpe yissrmaggg 61 qkvcyieghr vinlanemfg yngwahsitq qnvdfvdlnn gkfyvgvcaf vrvqlkdgsy 121 hedvgygvse glkskalsle karkeavtdg lkralrsfgn algncildkd ylrslnklpr 181 qlplevdltk akrqdlepsv eearynscrp nmalghpqlq qvtspsrpsh avipadqdcs 241 srslsssave seathqrklr qkqlqqqfre rmekqqvrvs tpsaekseaa ppappvthst 301 pvtvseplle kdflagvtqe liktlednse kwavtpdagd gvvkpssrad paqtsdtlal 361 nnqmvtqnrt phsvchqkpq aksgswdlqt ysadqrttgn weshrksqdm kkrkydpsy
- 特异性
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein. Cross reactivity with RAD52 protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52 protein.
- Top Product
- Discover our top product RAD52 Primary Antibody
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anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 269-418) antibody
RAD52 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5E11E7 unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Internal Region) antibodyRAD52 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyRAD52 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyRAD52 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Thr56) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 70-119) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 125-418) antibodyRAD52 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyRAD52 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 36-0) antibodyRAD52 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 70-119), (pTyr104) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This affinity purified antibody has been tested for use in Western blotting (1: 500-1: 2,000)using HeLa nuclear extracts. The antibody is also functional by ELISA (1: 5,000-1: 20,000). Dilutions for Western blotting represent a starting point dilution and further optimizationmay be required. The antibody detects a band of approximately 63.0 kDa (predictedmolecular weight: 46.3 kDa). Specific band detection by Western blot is blocked bypeptide competition by pre-incubating the antibody with the immunizing peptide prior toreaction with the membrane. Reactivity in other immunoassays is unknown.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
- 别名
- RAD52 (RAD52 产品)
- 别名
- Rad52yh antibody, RAD52 antibody, zgc:112400 antibody, LOC100223085 antibody, MGC115453 antibody, RAD52 homolog, DNA repair protein antibody, RAD52 homolog (S. cerevisiae) antibody, RAD52 homolog, DNA repair protein S homeolog antibody, recombinase antibody, DNA repair and recombination protein, putative antibody, RAD52 protein antibody, RAD52 antibody, Rad52 antibody, rad52 antibody, rad52.S antibody
- 背景
- The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).Synonyms: DNA repair protein RAD52 homolog
- 基因ID
- 5893
- NCBI登录号
- NP_602296
- UniProt
- P43351
- 途径
- DNA Damage Repair
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