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CD72 抗体 (FITC)

CD72 适用: 小鼠 FACS 宿主: 小鼠 Monoclonal CT-72-1 FITC
产品编号 ABIN114239
发货至: 中国
  • 抗原 See all CD72 抗体
    CD72 (CD72 Molecule (CD72))
    适用
    • 46
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    小鼠
    宿主
    • 37
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD72 antibody is conjugated to FITC
    应用范围
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    Flow Cytometry (FACS)
    特异性
    This antibody reacts with the CD72 alloantigen CD72.1, a B-cell surface protein that is encoded by the Cd72a allele. CD72.1 is expressed on cells of the B cell lineage, except plasma cells3. Mouse strains expressing CD72.1 include C57L/-, C58/-, DBA/1, DBA/2, and SWR/J. Tissue Distribution by Flow Cytometry Analysis: (Representative Dot Plot) Mouse Strain Tested: DBA/2 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.25 μg/10e6 cells Isotypic Control: FITC Mouse IgG2a.
    克隆位点
    CT-72-1
    亚型
    IgG2a
    Top Product
    Discover our top product CD72 Primary Antibody
  • 应用备注
    Flow cytometry (For details please see protocols / specifity below).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 25 µg of antibody per 1 x 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    PBS, 0.09 % sodium azide (NaN3) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    CD72 (CD72 Molecule (CD72))
    别名
    CD72 (CD72 产品)
    别名
    CD72c antibody, Ly-19 antibody, Ly-32 antibody, Ly-m19 antibody, Lyb-2 antibody, CD72b antibody, LYB2 antibody, CD72 antigen antibody, CD72 molecule antibody, Cd72 antibody, CD72 antibody
    背景
    CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
    基因ID
    100504743
    NCBI登录号
    XP_003086403
    UniProt
    P21855
    途径
    BCR Signaling
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