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Keratin 5 (KRT5) 抗体

ABIN112348 产品详细信息, 供应商: Log in to see
抗原
  • ckii
  • wu:fb02h09
  • wu:fj02d02
  • wu:fk78b12
  • zfCKII
  • KRT75
  • CK5
  • DDD
  • DDD1
  • EBS2
  • K5
  • KRT5A
  • 3300001P10Rik
  • AW146334
  • Krt2-5
  • Tfip8
  • Sak57
  • CK-5
  • Keratin-5
  • keratin 71
  • keratin 5
  • KRT71
  • krt5
  • KRT5
  • Krt5
适用
猫, 犬, Hamster, 人, 小鼠, 猪, 兔, 大鼠
319
94
45
42
9
8
3
3
2
1
1
1
宿主
小鼠
218
111
5
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
13
9
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
2
1
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
264
191
180
111
104
47
40
12
10
9
8
4
3
2
1
1
Supplier
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免疫原 Cytokeratins from a human lung cancer cell line (MR21).
克隆位点 RCK102
亚型 IgG1
特异性 RCK102 is a Cytokeratin antibody reacting with Cytokeratin 5 and Cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.
交叉反应 (详细) Also reacts to: KRT8
纯化方法 Purified
别名 Cytokeratin 5 (KRT5 Antibody 摘要)
背景 Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
基因ID 3852
NCBI登录号 NP_000415
UniProt P13647
研究领域 Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
应用备注 RCK102 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the original vial at 2-8 °C. After reconstitution store undiluted (in aliquots) at -20 °C.
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Keratin 5 (KRT5) 抗体 (ABIN112348) RCK102 Cytokeratin staining of a human squamous cell carcinoma of the lung.
背景 Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Kiani, Siyal: "Dimensions of urban growth in Pakistan." in: Pakistan development review, Vol. 30, Issue 4 Pt 2, pp. 681-91, 1992 (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Broers, Carney, Klein Rot, Schaart, Lane, Vooijs, Ramaekers: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, 1987 (PubMed).