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山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed

Name: 山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed
SKU: ABIN965007
Availability: OutOfStock

WB, FLISA, FM, DB Polyclonal IgG Atto 647N

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) . (山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed)

Immunofluorescence (IF) image for Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed (ABIN965007) (山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed)

2 images

(5 references)

产品编号 ABIN965007

Supplier Product No.: 612-156-120s

发货至: 中国

Datasheet as PDF

抗原 See all IgG products

IgG
抗原表位
2397

515

417

146

129

128

67

29

28

28

21

9

5

5

2

1

1
Heavy & Light Chain
适用
730

696

599

464

403

242

211

175

145

140

116

109

107

101

82

62

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
大鼠
宿主
1761

1556

567

258

122

112

40

32

22

21

15

11

11
山羊
克隆类型
3423

179

16

1
多克隆
标记
624

421

421

378

274

262

235

118

106

72

67

64

61

58

58

50

44

43

40

39

39

37

35

35

32

31

31

30

29

29

29

29

29

28

28

28

28

28

28

28

27

27

26

26

25

21

21

19

17

17

16
Atto 647N
应用范围
2092

2018

1883

1837

998

919

895

487

423

234

234

149

98

98

84

56

51

33

30

28

25

20

15

11

9

8

7

5

5

4

4

4

3

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.

612-156-120s

Supplier

Rockland

原理

Rat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed

交叉反应（详细）

Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.

产品特性

Anti-Rat IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

纯化方法

Rat IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Optional[Immunogen]: Rat IgG whole molecule

亚型

IgG
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Rabbit anti-Mouse IgG antibody (HRP) - Preadsorbed
ELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment

Goat anti-Rat IgG antibody (DyLight 488)
WB, FLISA, FM Polyclonal IgG DyLight 488

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG antibody (Cy3) - Preadsorbed
WB, FACS, FLISA, FM, DB Polyclonal IgG Cy3

Goat anti-Rat IgG (Heavy Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated

Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Rat IgG (Heavy Chain) antibody (beta-Gal)
ELISA, IHC, WB Polyclonal IgG beta-Gal

应用备注

Application Note: Anti-Rat IgG (H&L) conjugated to ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Meissner, Grimm, Johnston, Sutcliffe, Ng, Jefferis, Cachero, Lavis, Malkesman: "Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons." in: PLoS ONE, Vol. 13, Issue 8, pp. e0200759, (2019) (PubMed).

Dolan, Frechter, Bates, Dan, Huoviala, Roberts, Schlegel, Dhawan, Tabano, Dionne, Christoforou, Close, Sutcliffe, Giuliani, Li, Costa, Ihrke, Meissner, Bock, Aso, Rubin, Jefferis: "Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body." in: eLife, Vol. 8, (2019) (PubMed).

Turner-Evans, Wegener, Rouault, Franconville, Wolff, Seelig, Druckmann, Jayaraman: "Angular velocity integration in a fly heading circuit." in: eLife, Vol. 6, (2018) (PubMed).

Wolff, Rubin: "Neuroarchitecture of the Drosophila central complex: A catalog of nodulus and asymmetrical body neurons and a revision of the protocerebral bridge catalog." in: The Journal of comparative neurology, Vol. 526, Issue 16, pp. 2585-2611, (2018) (PubMed).

von Reyn, Nern, Williamson, Breads, Wu, Namiki, Card: "Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response." in: Neuron, Vol. 94, Issue 6, pp. 1190-1204.e6, (2017) (PubMed).
抗原

IgG

Abstract

IgG 产品

物质类

Antibody

背景

Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.