小鼠 anti-人 IgA Secretory Component Antibody
FACS, IP, WB, IHC, ELISA
Monoclonal
SC-05
IgG1 kappa
unconjugated
产品编号 ABIN7865653
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 小鼠 anti-人 IgA Secretory Component Antibody (ABIN7865653)
抗原
IgA Secretory Component
克隆类型
单克隆
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), ELISA
克隆位点
SC-05
亚型
IgG1 kappa
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适用
- 人, 大鼠
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宿主
- 小鼠
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原理
- anti-Human IgA Secretory Component mouse monoclonal, SC-05, purified
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纯化方法
- affinity chromatography
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免疫原
- affinity purified human secretory component
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Mouse anti-Human IgA Secretory Component antibody
FACS, IF, IHC (f) Monoclonal ECM1-792 IgG1 kappa unconjugated
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应用备注
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FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- IgA Secretory Component
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背景
- SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05. Positive control: Stomach, lung, or breast tumor.
抗原
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