小鼠 anti-人 IgG4 (pFc' Region) Antibody
Quick Overview for 小鼠 anti-人 IgG4 (pFc' Region) Antibody (ABIN7674790)
抗原
克隆类型
应用范围
克隆位点
亚型
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抗原表位
- pFc' Region
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适用
- 人
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宿主
- 小鼠
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原理
- Mouse MAb anti Human IgG4 (pFc subclass specific). In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.
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特异性
- Mouse MAb anti Human IgG4 (pFc subclass specific). In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.
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交叉反应 （详细）
- The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species
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产品特性
- The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc portion of the IgG4 Molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.`
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纯化方法
- Delipidated, heat inactivated ascites
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免疫原
- Highly purified monoclonal IgG4 isolated from pooled human serum
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Rabbit anti-Human IgG4 (AA 222-327) antibody
WB, IHC, IP, IF/ICC Polyclonal unconjugated
Mouse anti-Human IgG4 (Fc Region) antibody (PE)ELISA Monoclonal HP6025 IgG1 PE
Mouse anti-Human IgG4 (pFc' Region) antibody (Alkaline Phosphatase (AP))WB, IHC, ELISA Monoclonal HP6023 IgG3 Alkaline Phosphatase (AP)
Mouse anti-Human IgG4 (Fc Region) antibody (HRP)WB, IHC, ELISA Monoclonal HP6025 IgG1 HRP
Mouse anti-Human IgG4 (pFc' Region) antibody (Biotin)ELISA Monoclonal HP6023 IgG3 Biotin
Mouse anti-Human IgG4 (pFc' Region) antibody (FITC)ELISA Monoclonal HP6023 IgG3 FITC
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应用备注
- Immunohistochemistry, immunocytochemistry, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 1.0 mL sterile distilled water to obtain an IgG concentration of 1.0 mg/mL.
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储存条件
- 4 °C,-20 °C
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储存方法
- Lyophilized - can be stored at +4°C. After reconstitution aliquots can be stored at -20C. The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
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- IgG4
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物质类
- Antibody
抗原
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