Recombinant 小鼠 anti-兔 IgG Antibody (HRP)
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- 抗原 See all IgG products
- IgG
- 抗体类型
- Recombinant Antibody
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适用
- 兔
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宿主
- 小鼠
- Expression System
- Phage display
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克隆类型
- Multiclonal
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标记
- HRP
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- recombinant Multiclonal anti-rabbit IgG
- 特异性
- This is an antibody developed by antibody phage display technology using a human naive antibody gene library and rabbit IgG antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.
- 无交叉反应
- 山羊, 人, 小鼠
- 交叉反应 （详细）
- No known cross reactivity. No cross reactivtiy to mouse IgG, human IgG, goat IgG. Other species were not tested.
- 产品特性
- Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all subclasses of rabbit IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.
- 纯化方法
- Protein A purification
- 质量等级
- Animal-Free
- 免疫原
- No immunization, animal-free antibody development
- 亚型
- IgG2a
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- 应用备注
- Western Blot: 1:1,000 - 1:20,000 ELISA: 1:1,000 - 1:50,000 Optimal working dilution should be determined by the investigator
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, 0,02 % ProClin300
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 分子量
- 150 kDa
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