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Quick Overview for 小鼠 anti-人 IgE Antibody (APC) (ABIN7505983)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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原理
- Anti-Hu IgE APC
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产品特性
- The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
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纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Purified human IgE.
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Mouse anti-Human IgE antibody
ELISA, FACS Monoclonal 4G7-325 IgG unconjugated
Mouse anti-Human IgE antibody (FITC)FACS Monoclonal BE5 IgG1 FITC
Mouse anti-Human IgE antibody (Biotin)ELISA, FACS Monoclonal BE5 IgG1 Biotin
Mouse anti-Human IgE antibody (PE)FACS Monoclonal BE5 IgG1 PE
Mouse anti-Human IgE antibodyELISA, FACS Monoclonal BE5 IgG1 unconjugated
Mouse anti-Human IgE antibodyELISA, WB, FACS Monoclonal 4H10 IgG1 unconjugated
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应用备注
- Flow cytometry: Recommended dilution: 0.5-3 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze,Protect from prolonged exposure to light
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- IgE
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背景
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
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基因ID
- 35
抗原
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