Recombinant Alpaca anti-小鼠 IgG2 Antibody
WB
Monoclonal
14A4
Recombinant Antibody
unconjugated
single-domain Antibody (sdAb)
Quick Overview for Recombinant Alpaca anti-小鼠 IgG2 Antibody (ABIN7272951)
抗原
IgG2
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
克隆类型
单克隆
应用范围
Western Blotting (WB)
克隆位点
14A4
-
-
适用
- 小鼠
-
宿主
- Alpaca
-
Expression System
- E.coli
-
特异性
- Mouse IgG2, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
-
纯化方法
- Produced in: E.coli
-
免疫原
- sdAb anti-Mouse IgG2a/b binds to mouse-IgG2a and IgG2b subtype specifically and with high affinity. anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C
-
缓冲液
- sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.
-
储存条件
- 4 °C/-20 °C/-80 °C
-
储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
-
-
- IgG2
-
物质类
- Antibody
-
背景
- Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
抗原
-
You are here:
