Recombinant Alpaca anti-小鼠 IgG2 Antibody (Alexa Fluor 647)
WB, IHC, IF, ICC, FACS
Monoclonal
14A4
Recombinant Antibody
Alexa Fluor 647
single-domain Antibody (sdAb)
产品编号 ABIN7272925
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Recombinant Alpaca anti-小鼠 IgG2 Antibody (Alexa Fluor 647) (ABIN7272925)
抗原
IgG2
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
克隆类型
单克隆
标记
Alexa Fluor 647
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
克隆位点
14A4
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适用
- 小鼠
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宿主
- Alpaca
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Expression System
- E.coli
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原理
- Camelid sdAb anti-Mouse IgG2a/b Alexa Fluor™ 647, Clone(s) 14A4
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特异性
- Mouse IgG2, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
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纯化方法
- Produced in: E.coli
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免疫原
- This camelid sdAB anti-Mouse IgG2 is a species-specific antibody directed against IgG2a and IgG2b subtype from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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应用备注
- 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
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缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
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储存条件
- -20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- IgG2
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物质类
- Antibody
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背景
- In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
抗原
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