Recombinant Alpaca anti-小鼠 IgG2 Antibody (Alexa Fluor 568)
WB, IHC, ICC, IF, FACS
Monoclonal
14A4
Recombinant Antibody
Alexa Fluor 568
single-domain Antibody (sdAb)
产品编号 ABIN7272924
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Recombinant Alpaca anti-小鼠 IgG2 Antibody (Alexa Fluor 568) (ABIN7272924)
抗原
IgG2
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
克隆类型
单克隆
标记
Alexa Fluor 568
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
克隆位点
14A4
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适用
- 小鼠
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宿主
- Alpaca
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Expression System
- E.coli
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特异性
- Mouse IgG2, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
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纯化方法
- Produced in: E.coli
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免疫原
- FluoTag-X2 anti-Mouse IgG2 is a species-specific FluoTag directed against IgG2a and IgG2b subtype from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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应用备注
- 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
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缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
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储存条件
- -20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- IgG2
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物质类
- Antibody
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背景
- In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
抗原
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