山羊 anti-大鼠 IgG2ab, Subclass Specific (Subclass specific) Antibody - Preadsorbed
ELISA, DB, WB, IFA
Polyclonal
IgG
unconjugated
产品编号 ABIN458751
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-大鼠 IgG2ab, Subclass Specific (Subclass specific) Antibody - Preadsorbed (ABIN458751)
抗原
IgG2ab
(IgG2ab, Subclass Specific)
克隆类型
多克隆
应用范围
ELISA, Dot Blot (DB), Western Blotting (WB), Indirect Immunofluorescence Assay (IFA)
亚型
IgG
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抗原表位
- Subclass specific
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适用
- 大鼠
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宿主
- 山羊
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原理
- Goat anti Rat IgG2ab (subclass specific)
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特异性
- IgG fraction of polyclonal Goat antiSerum to Rat IgG2ab, subclass specific
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交叉反应 （详细）
- This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.
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产品特性
- The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested by immunoelectrophoresis and double radial immunodiffusion against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, gM and IgA or any non-Ig protein in rat serum. As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized IgG (7S) is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- IgG2ab (IgG2ab, Subclass Specific)
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别名
- IgG2ab
抗原
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