山羊 anti-大鼠 IgG2ab, Subclass Specific (Subclass specific) Antibody (Biotin)
ICC, ELISA, DB, WB, IHC (p)
Polyclonal
Biotin
产品编号 ABIN1099729
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-大鼠 IgG2ab, Subclass Specific (Subclass specific) Antibody (Biotin) (ABIN1099729)
抗原
IgG2ab
(IgG2ab, Subclass Specific)
克隆类型
多克隆
标记
Biotin
应用范围
Immunocytochemistry (ICC), ELISA, Dot Blot (DB), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- Subclass specific
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适用
- 大鼠
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宿主
- 山羊
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原理
- Goat anti Rat IgG2ab (subclass specific), conjugated with Biotin
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特异性
- Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG2ab, subclass specific.
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交叉反应 （详细）
- This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this immunoconjugate has not been tested in detail.
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产品特性
- The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified
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免疫原
- Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- IgG2ab (IgG2ab, Subclass Specific)
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别名
- IgG2ab
抗原
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