兔 anti-小鼠 IgG2 Antibody (TRITC) - Preadsorbed
IHC, ICC, IF
Polyclonal
IgG
TRITC
Quick Overview for 兔 anti-小鼠 IgG2 Antibody (TRITC) - Preadsorbed (ABIN458468)
抗原
IgG2
克隆类型
多克隆
标记
TRITC
应用范围
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
亚型
IgG
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适用
- 小鼠
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宿主
- 兔
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特异性
- Specificity of this product has not been tested in detail.
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纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
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免疫原
- Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
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In immunocytochemical and immunohistochemical single and double staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, as indicating second antibody conjugate to analyze or separate human cells after reacting with monoclonal mouse antibodies in immunofluorescence microscopy, fluorescence activated cell sorting and flow cytometry, to identify and measure IgG2 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
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浓度
- 10 mg/mL
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缓冲液
- TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgG2
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物质类
- Antibody
抗原
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