兔 anti-人 Ig (Chain kappa), (Free & Bound) Antibody
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北京 101111
Quick Overview for 兔 anti-人 Ig (Chain kappa), (Free & Bound) Antibody (ABIN458112)
抗原
克隆类型
应用范围
亚型
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抗原表位
- Chain kappa, Free & Bound
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适用
- 人
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宿主
- 兔
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特异性
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The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasmaPrecipitating polyclonal rabbit antiserum to free and bound human Ig kappa light chain
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion. -
产品特性
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To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.
Physicochemical characteristic: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human serum -
纯化方法
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Delipidated, heat inactivated lyophilized whole serum.
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with human serum proteins. -
免疫原
- A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.
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缓冲液
- No foreign proteins added.
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储存液
- Without preservative
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注意事项
- Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Repeated thawing and freezing should be avoided.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Ig
抗原
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