The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasmaPrecipitating polyclonal rabbit antiserum to free and bound human Ig lambda light chain To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.
产品特性
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion. Physicochemical characteristic: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human serum
纯化方法
Delipidated, heat inactivated lyophilized whole serum. Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with human serum proteins.
免疫原
A pool of purified Bence Jones lambda proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
ELISA, IHC, WB, IEP, IM, DB
Polyclonal
Biotin
F(ab')2 fragment
应用备注
Optimal working dilutions should be determined experimentally by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.
缓冲液
No foreign proteins added.
储存液
Without preservative
注意事项
Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Repeated thawing and freezing should be avoided.
储存条件
4 °C/-20 °C
储存方法
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.