小鼠 anti-人 IgG3 (Allotype G3m(U)) Antibody
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北京 101111
Quick Overview for 小鼠 anti-人 IgG3 (Allotype G3m(U)) Antibody (ABIN457932)
抗原
克隆类型
应用范围
克隆位点
亚型
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抗原表位
- Allotype G3m(U)
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适用
- 人
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宿主
- 小鼠
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特异性
- Monoclonal Mouse antibody to Human IgG3m(u), allotype specific.
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交叉反应 （详细）
- The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
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产品特性
- The reactivity of the antiserum is restricted to an allotype specific determinant of the IgG3m(u) molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3m(u), and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
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纯化方法
- Delipidated, heat inactivated, stable ascites
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过滤
- Heat-inactivated
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免疫原
- Highly purified monoclonal IgG3m(u) isolated from human serum
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Mouse anti-Human IgG3 (Hinge Region) antibody (PE)
ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal HP6050 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
Mouse anti-Human IgG3 (Hinge Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6050 IgG1 HRP
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应用备注
- Indirect immunofluorescence,ELISA.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/mL. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/mL. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- RT,4 °C,-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
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- IgG3
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物质类
- Antibody
抗原
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