小鼠 anti-人 IgG3 (Chain kappa) Antibody
Quick Overview for 小鼠 anti-人 IgG3 (Chain kappa) Antibody (ABIN457931)
抗原
克隆类型
应用范围
克隆位点
亚型
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抗原表位
- Chain kappa
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG3, subclass specific
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纯化方法
- Delipidated, heat inactivated lyophilized stable ascites
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免疫原
- Highly purified monoclonal IgG1 kappa isolated from pooled human serum
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Mouse anti-Human IgG3 (Hinge Region) antibody
ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 unconjugated
Mouse anti-Human IgG3 (Hinge Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal HP6050 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
Mouse anti-Human IgG3 (Hinge Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6050 IgG1 HRP
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应用备注
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To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
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浓度
- 1.0 mg/mL
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缓冲液
- No foreign proteins added.
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储存液
- Without preservative
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgG3
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物质类
- Antibody
抗原
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