小鼠 anti-人 IgA2 (Allotype IgA2(m)2) Antibody
Quick Overview for 小鼠 anti-人 IgA2 (Allotype IgA2(m)2) Antibody (ABIN457924)
抗原
克隆类型
应用范围
克隆位点
亚型
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抗原表位
- Allotype IgA2(m)2
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适用
- 人
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宿主
- 小鼠
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特异性
- Monoclonal Mouse antiSerum to Human IgA2(m)2, allotype specific
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交叉反应 （详细）
- The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
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产品特性
- The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
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纯化方法
- Delipidated, heat inactivated, stable ascites
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过滤
- Heat-inactivated
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免疫原
- Highly purified monoclonal IgA2(m)2 isolated from human serum.
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Mouse anti-Human IgA2 (Fc Region) antibody
ELISA Monoclonal A9604D2 IgG1 unconjugated
Mouse anti-Human IgA2 antibody (PE)ELISA Monoclonal A9604D2 IgG1 PE
Mouse anti-Human IgA2 antibody (FITC)ELISA Monoclonal A9604D2 IgG1 FITC
Mouse anti-Human IgA2 (Fc Region) antibody (Biotin)ELISA Monoclonal A9604D2 IgG1 Biotin
Mouse anti-Human IgA2 (Fc Region) antibody (HRP)WB, ELISA, IHC Monoclonal A9604D2 IgG1 HRP
Mouse anti-Human IgA2 (Fc Region) antibody (Alkaline Phosphatase (AP))WB, ELISA, IHC Monoclonal A9604D2 IgG1 Alkaline Phosphatase (AP)
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应用备注
- Indirect immunofluorescence,ELISA.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added.
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储存条件
- RT,4 °C,-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
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- IgA2
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别名
- IgA22
抗原
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