小鼠 anti-人 IgG3 Antibody (Biotin)
Quick Overview for 小鼠 anti-人 IgG3 Antibody (Biotin) (ABIN457895)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Biotin-conjugated purified monoclonal mouse antibody to human IgG3, subclass specific
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产品特性
- Biotin/IgG protein molar ratio (B/P) approximately 2.2.
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纯化方法
- Purified
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免疫原
- Highly purified monoclonal IgG3 isolated from pooled human serum.
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Mouse anti-Human IgG3 (Hinge Region) antibody (PE)
ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal HP6050 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
Mouse anti-Human IgG3 (Hinge Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6050 IgG1 HRP
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应用备注
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To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250, in ELISA from 1:100 upwards, in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存液
- Without preservative
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgG3
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物质类
- Antibody
抗原
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