小鼠 anti-人 IgG3 Antibody (HRP)
Quick Overview for 小鼠 anti-人 IgG3 Antibody (HRP) (ABIN457838)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to the subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG3, subclass specific
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产品特性
- Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
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纯化方法
- Purified
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免疫原
- Highly purified monoclonal IgG3 isolated from pooled human serum
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Mouse anti-Human IgG3 (Hinge Region) antibody
ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 unconjugated
Mouse anti-Human IgG3 (Hinge Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal HP6050 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
Mouse anti-Human IgG3 (Hinge Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6050 IgG1 HRP
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应用备注
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To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存液
- Without preservative
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注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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- IgG3
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物质类
- Antibody
抗原
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