ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
特异性
Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
灵敏度
Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
产品特性
IGHE, chromosome 14q32.33
纯化方法
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
纯度
> 85 %, tested by analytical Gel Permeation Chromatography
When used for functional cellular assays at dilutions < 1:100, the Na-azide free formulation (Cat. No. 0910-1-010 / 050-B) is recommended
限制
仅限研究用
浓度
1 mg/mL
缓冲液
PBS, pH=7.2, 0.1 % NaN3
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.