ELISA, IHC, WB, DB
Polyclonal
Alkaline Phosphatase (AP)
应用备注
Suitable for Immunoblotting (Western or Dot blot) (1/1,000-1/5,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistryas well as other peroxidase-antibody based enzymatic assays requiring lot-to-lotconsistency.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1.0 mL of deionized water (or equivalent).
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
储存液
Thimerosal (Merthiolate)
注意事项
Biohazard Informations: This product contains thimerosal which is hazardous.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.