山羊 anti-雪貂 IgM (Chain mu) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
ELISA, IHC, WB, DB
Polyclonal
Alkaline Phosphatase (AP)
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- 抗原 See all IgM products
- IgM
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抗原表位
- Chain mu
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适用
- 雪貂
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
- Ferret IgM µ chain
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- 应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Ferret IgM in astandard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,500 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C.
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Synonyms: Ferret Immunoglobulin M
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