Protein A Protein (HRP)
This Native Protein A protein is expressed in Staphylococcus aureus.
Quick Overview for Protein A Protein (HRP) (ABIN967967)
抗原
See all Protein A 蛋白
Protein A
蛋白类型
Native
宿主
Staphylococcus aureus
资源
Staphylococcus aureus
应用范围
Western Blotting (WB)
-
-
标记
- This Protein A protein is labelled with HRP.
-
产品特性
-
Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes. It can also be utilized for antibody purification. As a horseradish peroxidase conjugate, protein A can be used instead of secondary antibody detection methods in Western blotting with polyclonal antibodies and with IgG2a and IgG2b mouse monoclonal antibodies.
Horseradish peroxidase (HRP) was coupled to protein A at a concentration of 4 mg/mg of protein A using the periodate method. For the detection of antigen using polyclonal antibodies in Western blotting, a concentration of 0.5 µg/ml (1:2000 dilution) is suggested. -
纯化方法
- Purified from tissue culture supernatant or ascites by affinity chromatography.
-
-
Protein A (AA 37-469) protein
宿主: Staphylococcus aureus 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
-
-
说明
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- 50 % glycerol, 20 mM sodium phosphate pH 7.5, 150 mM NaCl.
-
储存条件
- -20 °C
-
储存方法
- Store undiluted at -20° C.
-
-
- Protein A
-
别名
- ProteinA
抗原
-
You are here:
