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Protein A Protein (Biotin)

宿主: Staphylococcus aureus ELISA, WB, DB, IHC
产品编号 ABIN1536532
发货至: 中国
  • 抗原 See all Protein A 蛋白
    Protein A
    宿主
    Staphylococcus aureus
    宿主
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    标记
    This Protein A protein is labelled with Biotin.
    应用范围
    ELISA, Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC)
    Top Product
    Discover our top product Protein A 蛋白
  • 应用备注
    Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:5,000-1:50,000 Western blot: 1:5,000-1:50,000 ELISA: 1:50,000-1:500,000 Other: user-optimized.
    限制
    仅限研究用
  • 浓度
    2 mg/ml
    缓冲液
    PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.
  • Wen, He, Lee: "Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).

  • 抗原
    Protein A
    Abstract
    Protein A 产品
    别名
    alpha protein Protein, repB Protein
    背景
    Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
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