Protein G Protein (Alkaline Phosphatase (AP))
Quick Overview for Protein G Protein (Alkaline Phosphatase (AP)) (ABIN964531)
抗原
See all Protein G 蛋白蛋白类型
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应用范围
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标记
- This Protein G protein is labelled with Alkaline Phosphatase (AP).
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Supplier Product No.
- pg00-05
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Supplier
- Rockland
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特异性
- Protein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A.
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Protein G protein (His tag)
宿主: Streptococcus 宿主: 大肠杆菌（E. Coli） Recombinant >98 % as determined by SDS-PAGE.
Protein G protein (Biotin)宿主: Streptococcus Recombinant ELISA, WB, IHC
Protein G protein宿主: Streptococcus 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, IP
Protein G protein宿主: Streptococcus Recombinant Con
Protein G protein (FITC)宿主: Streptococcus Recombinant FACS, IM
Protein G protein (HRP)宿主: Streptococcus Recombinant ELISA, WB
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应用备注
- Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
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说明
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Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- Protein G
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背景
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Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. Protein G also binds to albumin. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments. Protein G Alkalline Phosphatase Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling.
Synonyms: ProG, Streptococcus G protein, ALP, Alk Phos
抗原
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