Protein G Protein (HRP)
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- 抗原 See all Protein G 蛋白
- Protein G
- 宿主
- Streptococcus
- 宿主
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- 标记
- This Protein G protein is labelled with HRP.
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoelectron Microscopy (IEM), Dot Blot (DB)
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- Discover our top product Protein G 蛋白
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Protein G protein (His tag)
宿主: Streptococcus 宿主: 大肠杆菌（E. Coli） Recombinant >98 % as determined by SDS-PAGE.
Protein G protein宿主: Streptococcus 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, IP
Protein G protein宿主: Streptococcus Recombinant Con
Protein G protein (FITC)宿主: Streptococcus Native FACS, IM
Protein G protein (HRP)宿主: Streptococcus Native ELISA, WB
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- 应用备注
- Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including primary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Dot blot: 1:2,000-1:10,000 Western blot: 1:2,000-1:10,000 ELISA: 1:10,000-1:50,000 Immunohistology: 1:1,000-1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized power with 1.0 ml of deionized water to a final HRP-Protein G concentration of 1 mg/ml.
- 缓冲液
- PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- HRP-Protein G should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freezing and thawing cycles.
- 有效期
- 24 months
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- 抗原
- Protein G
- Abstract
- Protein G 产品
- 背景
- Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry.
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