Protein G Protein (HRP)
Quick Overview for Protein G Protein (HRP) (ABIN964529)
抗原
See all Protein G 蛋白蛋白类型
宿主
宿主
应用范围
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标记
- This Protein G protein is labelled with HRP.
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Supplier Product No.
- pg00-03
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Supplier
- Rockland
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特异性
- Protein G Peroxidase was prepared from chromatographically pure Protein G. Protein G Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A.
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Protein G protein (His tag)
宿主: Streptococcus 宿主: 大肠杆菌（E. Coli） Recombinant >98 % as determined by SDS-PAGE.
Protein G protein (Biotin)宿主: Streptococcus Recombinant ELISA, WB, IHC
Protein G protein宿主: Streptococcus 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, IP
Protein G protein宿主: Streptococcus Recombinant Con
Protein G protein (FITC)宿主: Streptococcus Recombinant FACS, IM
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应用备注
- Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein GA Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.
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说明
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Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein GA Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 1.0 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存条件
- 4 °C
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有效期
- 12 months
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- Protein G
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背景
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Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. Protein G also binds to albumin. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Synonyms: HRP, peroxidase, Horseradish peroxidase protein G
抗原
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