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Protein A Protein (Sepharose)

This Native Protein A protein is expressed in not_set.
Rockland
产品编号 ABIN964524
Supplier Product No.: pa50-00-0002
发货至: 中国
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Quick Overview for Protein A Protein (Sepharose) (ABIN964524)

抗原

See all Protein A 蛋白
Protein A

蛋白类型

Native

宿主

Staphylococcus aureus

宿主

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应用范围

Immunoprecipitation (IP)
  • 标记

    This Protein A protein is labelled with Sepharose.

    Supplier Product No.

    pa50-00-0002

    Supplier

    Rockland

    产品特性

    Concentration Definition: drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
    Performance Data: Bead size 45-165 µm in size when swollen. ~35 mg Human IgG bound per cc of drained beads.
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  • 应用备注

    Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

    说明

    Sepharose 4 Fast Flow from GE/Amersham-Pharmacia

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    6.0 mg/cc

    缓冲液

    20% (v/v) Ethanol

    储存条件

    4 °C
  • 抗原

    Protein A

    背景

    Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
    Synonyms: Sepharose Protein A
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