Protein A Protein (Sepharose)
This Native Protein A protein is expressed in .
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Quick Overview for Protein A Protein (Sepharose) (ABIN964524)
抗原
See all Protein A 蛋白
Protein A
蛋白类型
Native
宿主
Staphylococcus aureus
宿主
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应用范围
Immunoprecipitation (IP)
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标记
- This Protein A protein is labelled with Sepharose.
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Supplier Product No.
- pa50-00-0002
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Supplier
- Rockland
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产品特性
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Concentration Definition: drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
Performance Data: Bead size 45-165 µm in size when swollen. ~35 mg Human IgG bound per cc of drained beads.
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Protein A (AA 37-469) protein
宿主: Staphylococcus aureus 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
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应用备注
- Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
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说明
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Sepharose 4 Fast Flow from GE/Amersham-Pharmacia
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 6.0 mg/cc
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缓冲液
- 20% (v/v) Ethanol
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储存条件
- 4 °C
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- Protein A
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背景
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Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
Synonyms: Sepharose Protein A
抗原
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