Quick Overview for Avidin Protein (AVD) (Glucose Oxidase) (ABIN964122)
抗原
See all Avidin (AVD) 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This Avidin protein is labelled with Glucose Oxidase.
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Supplier Product No.
- a003-07
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Supplier
- Rockland
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原理
- Avidin Glucose Oxidase Conjugated
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纯化方法
- Avidin Glucose Oxidase was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and an
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过滤
- Sterile filtered
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated has been tested by ELISA and can be utilized in both Western Blotting and ELISA experiment formats in combinati
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:8,000 - 1:32,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 1.0 mL)
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Avidin (AVD)
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别名
- Avidin
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背景
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Synonyms: AvD, Avidin Biotin Complex, GOD, GOx, Avidin Glucose Oxidase Conjugated
Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the grea
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UniProt
- P02701
抗原
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