Quick Overview for Avidin Protein (AVD) (Glucose Oxidase) (ABIN964122)
抗原
See all Avidin (AVD) 蛋白
Avidin (AVD)
宿主
Chicken egg
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应用范围
ELISA, Western Blotting (WB)
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标记
- This Avidin protein is labelled with Glucose Oxidase.
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Supplier Product No.
- a003-07
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Supplier
- Rockland
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特异性
- Avidin Glucose Oxidase was prepared from chromatographically pure avidin followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Glucose Oxidase.
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过滤
- Sterile filtered
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应用备注
- Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
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说明
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Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- Avidin (AVD)
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别名
- Avidin
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背景
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Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Avidin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Synonyms: AvD, Avidin Biotin Complex, GOD, GOx
抗原
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