Quick Overview for Avidin Protein (AVD) (HRP) (ABIN964120)
抗原
See all Avidin (AVD) 蛋白
Avidin (AVD)
宿主
Chicken egg
宿主
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应用范围
ELISA, Western Blotting (WB)
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标记
- This Avidin protein is labelled with HRP.
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Supplier Product No.
- a003-03
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Supplier
- Rockland
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特异性
- Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase.
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应用备注
- Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
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说明
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Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Gentamicin sulfate
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注意事项
- Do NOT add Sodium Azide!
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储存条件
- 4 °C
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有效期
- 12 months
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- Avidin (AVD)
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别名
- Avidin
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背景
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Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Avidin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Synonyms: HRP, Horseradish peroxidase, AvD, Avidin Biotin Complex
抗原
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