Quick Overview for Avidin Protein (AVD) (TRITC) (ABIN964117)
抗原
See all Avidin (AVD) 蛋白
Avidin (AVD)
蛋白类型
Native
宿主
Chicken egg
资源
小鸡
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA
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标记
- This Avidin protein is labelled with TRITC.
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Supplier Product No.
- a003-00
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Supplier
- Rockland
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原理
- Avidin Rhodamine Conjugated
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纯化方法
- Avidin Rhodamine Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.
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应用备注
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Flow Cytometry Dilution: 1:500 - 1:2,500
Application Note: Avidin Rhodamine Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
FLISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 1.0 mL)
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Avidin (AVD)
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别名
- Avidin
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背景
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Synonyms: Avidin Rhodamine Conjugated, Avidin TRITC Conjugated
Background: Avidin Rhodamine Conjugated is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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UniProt
- P02701
抗原
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