Purified recombinant Human FGF acidic protein Expression System: E.coli Bioactivity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ? 0.5 ng/mL corresponding to a specific activity of ? 2 x 10^6 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ? 0.2 ng/mL in the presence of 10 µg/mL heparin, corresponding to a specific activity of ? 5 x 10^6 units/mg.
宿主: 大鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
< 95 % by SDS-PAGE gel and HPLC analyses.
Active
应用备注
Each Investigator should determine their own optimal working dilution for specific applications.
限制
仅限研究用
状态
Lyophilized
缓冲液
Supplied as a lyophilized powder.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
RT/-20 °C
抗原
FGF acidic
背景
FGF acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF acidic has the ability to signal through all the FGF receptors. Alternative Names: FGF-1 protein, ECGF-beta protein, HBGF-1 protein, Fibroblast Growth Factor-acidic protein