FGF acidic (AA 16-155) (Active) 蛋白
宿主: 小鼠
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Func
Active
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- 抗原
- FGF acidic
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 16-155
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func)
- 原理
- Recombinant Mouse Fibroblast Growth Factor 1/FGF-1/FGFa (Phe16-Asp155)
- 序列
- FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD
- 产品特性
- Recombinant Mouse Fibroblast Growth Factor Acidic is produced with our E. coli expression system. The target protein is expressed with sequence (Phe16-Asp155) of Mouse FGF-A.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
-
FGF acidic (AA 16-155) protein
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % as determined by SDS-PAGE.
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- 说明
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Biological activity: ED50 is less than 0.5 ng/ml as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of Heparin.Specific Activity of 2.0 x 106 IU/mg.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 500 mM NaCl, pH 6.6.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
-
- 抗原
- FGF acidic
- 背景
-
FGF acidic is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors.
Alternative Names: Fibroblast Growth Factor 1, FGF-1, Acidic Fibroblast Growth Factor, aFGF, Heparin-Binding Growth Factor 1, HBGF-1, Fgf1, Fgf-1, Fgfa - 分子量
- 15.7 kDa
- UniProt
- P61148
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