FGF acidic (Active) 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for FGF acidic (Active) 蛋白 (ABIN935349)
抗原
FGF acidic
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % pure
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序列
- MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
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产品特性
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Purified recombinant Human FGF acidic protein
Expression System: E.coli
Bioactivity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ? 10 ng/mL, corresponding to a specific activity of ? 1 x 10^5 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ? 0.5 ng/mL in the presence of 10 µg/mL heparin, corresponding to a specific activity of ? 2 x 10^6 units/mg. -
内毒素水平
- < 0.1 ng per μg (1 EU/μg).
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FGF acidic (AA 16-155) protein
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % as determined by SDS-PAGE.
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应用备注
- Each Investigator should determine their own optimal working dilution for specific applications.
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说明
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Biological activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is approx. 10 ng/mL, corresponding to a specific activity of approx. 1 x 10^5 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is approx. 0.5 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of approx. 2 x 10^6 units/mg.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 5 M Na3PO4
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缓冲液
- Lyophilized from 5 mM Na3PO4, pH 8.0 with 105 mM NaCl.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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- FGF acidic
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背景
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FGF acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF acidic has the ability to signal through all the FGF receptors.
Alternative Names: HBGF-1 protein, FGF-1 protein, Fibroblast Growth Factor-acidic protein, ECGF-beta protein -
分子量
- 15.8 kDa
抗原
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