Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491)
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- 抗原 See all Glucose-6-Phosphate Dehydrogenase (G6PD) 蛋白
- Glucose-6-Phosphate Dehydrogenase (G6PD)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-491
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宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
- 产品特性
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Purified recombinant E.coli G6 PD protein
Expression System: E.coli
Bioactivity: Specific activity is 8-10 units/mL obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 m D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C.
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 90 % pure
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- Discover our top product G6PD 蛋白
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Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-491) protein
G6PD 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Glucose-6-Phosphate Dehydrogenase (G6PD) protein (His tag)G6PD 宿主: 人 宿主: Hi-5 Cells Recombinant > 95 % by SDS - PAGE SDS
Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-515) protein (GST tag)G6PD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-491) protein (His tag)G6PD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glucose-6-Phosphate Dehydrogenase (G6PD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)G6PD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- G6PD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 实验流程
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- Prepare a 3 mL reaction cocktail into a suitable container: The final concentrations are 53 mM gylcylglycine, 3 mM D-glucose 6-phosphate, 0.63 mM beta-NADP, 13 mM MgCl2.
2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
3. Add 4 µg of recombinant G6PD into reaction cocktail and mix immediately.
4. Record the increase at A340 nm for 5 minutes.
- Prepare a 3 mL reaction cocktail into a suitable container: The final concentrations are 53 mM gylcylglycine, 3 mM D-glucose 6-phosphate, 0.63 mM beta-NADP, 13 mM MgCl2.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 50 mM MES 6.0, 0.0 mM PMSF, 0 mM EDTA, 0.0 mM DTT, and 10 % glycerol.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- Glucose-6-Phosphate Dehydrogenase (G6PD)
- 别名
- G6PD (G6PD 产品)
- 背景
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Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphogluconolactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: Glucose 6 phosphate 1 dehydrogenase protein, G6PD1 protein, zwf protein, GPD 6, Glucose-6-phosphate 1-dehydrogenase G6PD protein, Glucose-6-phosphate 1-dehydrogenase protein, G6PD, POS10 protein, GPD-6, GPD-6 protein, MET19 protein, GPD 6 protein, G6pdx protein, Zwf1p. - 分子量
- 55.7 kDa (491 AA)
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
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