Quick Overview for GSTM1 Protein (AA 1-218) (ABIN935016)
抗原
See all GSTM1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-218
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序列
- MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
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产品特性
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Purified recombinant Mouse GSTM1 protein
Expression System: E.coli
Bioactivity: Specific activity is 3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C.
Molecular weight on SDS-PAGE will appear higher. -
内毒素水平
- < 1.0 EU per µg of protein (determined by LAL method)
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Glutathione S-Transferase mu 1 (GSTM1) (AA 1-218) protein
GSTM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Glutathione S-Transferase mu 1 (GSTM1) (AA 1-181) protein (His tag)GSTM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85% by SDS-PAGE SDS
Glutathione S-Transferase mu 1 (GSTM1) (AA 1-181) protein (GST tag)GSTM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- GSTM1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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实验流程
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- Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
3.2 % (v/v) ethanol.
2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
3. Add 50 µL of recombinant mouse GSTM1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
4. Mix by inversion and record the increase at A340 nm for 5 minutes.
- Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as a liquid in PBS, pH 7.4, containing 5 mM glutathione.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))
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别名
- GSTM1
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背景
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GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GST class-mu1 protein, GSTM-1, GST class mu 1 protein, GSTM1, GSTM 1 protein, GSTM1., GST1 protein, GSTM-1 protein, GST class Mu protein, pmGT10 protein, Glutathione S-transferase Mu 1 Glutathione S transferase mu protein, GSTM 1, GST mu protein, GST 1-1 protein -
分子量
- 25.9 kDa (218 AA)
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途径
- Negative Regulation of Transporter Activity
抗原
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